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Biomaterials and Biomedical Engineering Volume 1, Number 3, September 2014 , pages 163-174 DOI: https://doi.org/10.12989/bme.2015.1.3.163 |
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Cross-linkable and water-soluble phospholipid polymer as artificial extracellular matrix |
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Eri Maeta and Kazuhiko Ishihara
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| Abstract | ||
| The objective of this study is to prepare an artificial extracellular matrix (ECM) for cell culture by using polymer hydrogels. The polymer used is a cytocompatible water-soluble phospholipid polymer: poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-n-butyl methacrylate-p-nitrophenyloxycarbonyl poly(ethylene oxide) methacrylate (MEONP)] (PMBN). The hydrogels are prepared using a cross-linking reaction between PMBN and diamine compounds, which can easily react to the MEONP moiety under mild conditions. The most favorable diamine is the bis(3-aminopropyl) poly(ethylene oxide) (APEO). The effects of cross-linking density and the chemical structure of cross-linking molecules on the mechanical properties of the hydrogel are evaluated. The storage modulus of the hydrogel is tailored by tuning the PMBN concentration and the MEONP/amino group ratio. The porous structure of the hydrogel networks depends not only on these parameters but also on the reaction temperature. We prepare a hydrogel with 40–50 | ||
| Key Words | ||
| phospholipid polymer; cytotoxicity; artificial extracellular matrix; hydrogel; cell culture | ||
| Address | ||
| Eri Maeta, Kazuhiko Ishihara: Department of Materials Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku,Tokyo 113-8656, Japan Kazuhiko Ishihara: Department of Bioengineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan | ||
