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Membrane Water Treatment Volume 10, Number 2, March 2019 , pages 121-125 DOI: https://doi.org/10.12989/mwt.2019.10.2.121 |
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Characterization of a protein-based filtering cartridge for the removal of atrazine-induced effects on living cultured cells |
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Giuseppina Basini, Francesca Grasselli, Simona Bussolati, Virna Conti, Francesco Bianchi, Stefano Grolli, Federica Bianchi and Roberto Ramoni
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| Abstract | ||
| Chronic exposure to atrazine (ATR) raises concerns about adverse effects on reproductive functions. We tested our previously validated filtering device, the OBP-based filter, onto a biological model constituted of cultured swine granulosa cells treated for 48 h with media conditioned with 0.1 or 10 micrometre ATR evaluating cell viability and steroidogenesis. The tested atrazine concentrations did not change granulosa cell viability and no filtering effects was observed following treatments with media prepared with differently filtered water. As for steroidogenesis, treatment of water with OBP -based filter containing 10 micrometre atrazine completely suppressed the stimulatory effect of 10 micrometre atrazine on progesterone production as well as the inhibitory effect of 0.1 micrometre ATR on estradiol-17 production by granulosa cells. Our data demonstrate that the impairment of steroidogenesis induced by ATR is effectively removed after water filtration in the experimental device thus suggesting potential use in biotechnological applications on living cells and/or organisms. | ||
| Key Words | ||
| atrazine; OBP; granulosa cells; steroidogenesis | ||
| Address | ||
| Giuseppina Basini, Francesca Grasselli, Simona Bussolati, Virna Conti, Stefano Grolli, Federica Bianchi and Roberto Ramoni: Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, Italy Federica Bianchi: Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università degli Studi di Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy | ||