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Membrane Water Treatment Volume 2, Number 2, April 2011 , pages 91-103 DOI: https://doi.org/10.12989/mwt.2011.2.2.091 |
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Separation and flux characteristics in cross-flow ultrafiltration of bovine serum albumin and bovine hemoglobin solutions |
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Ruey-Chang Hsiao, Chia-Lin Hung, Su-Hsia Lin and Ruey-Shin Juang
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| Abstract | ||
| The flux behavior in the separation of equimolar bovine serum albumin (BSA) and bovine hemoglobin (HB) in aqueous solutions by cross-flow ultrafiltration (UF) was investigated, in which polyacylonitrile membrane with a molecular weight cut-off (MWCO) of 100 kDa was used. BSA and HB have comparable molar mass (67,000 vs. 68,000) but different isoelectric points (4.7 vs. 7.1). The effects of process variables including solution pH (6.5, 7.1, and 7.5), total protein concentration (1.48 and 7.40 | ||
| Key Words | ||
| separation; bovine serum albumin; bovine hemoglobin; cross-flow ultrafiltration; specific cake resistance. | ||
| Address | ||
| Ruey-Chang Hsiao:Department of Chemical and Material Engineering, Lung Hwa University of Science and Technology, Taoyuan 333, Taiwan Chia-Lin Hung and Ruey-Shin Juang:Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li 32003, Taiwan Su-Hsia Lin:Department of Chemical and Materials Engineering, Nanya Institute of Technology, Chung-Li 320, Taiwan | ||