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Biomaterials and Biomedical Engineering
  Volume 3, Number 3, September 2016 , pages 129-140
 open access

Polymeric nanoparticles as dual-imaging probes for cancer management
Jyothi U. Menon, Parth Jadeja, Pranjali Tambe, Dheeraj Thakore, Shanrong Zhang, Masaya Takahashi, Zhiwei Xie, Jian Yang and Kytai T. Nguyen

    This article reports the development of biodegradable photoluminescent polymer (BPLP)-based nanoparticles (NPs) incorporating either magnetic nanoparticles (BPLP-MNPs) or gadopentate dimeglumine (BPLP-Gd NPs), for cancer diagnosis and treatment. The aim of the study is to compare these nanoparticles in terms of their surface properties, fluorescence intensities, MR imaging capabilities, and in vitro characteristics to choose the most promising dual-imaging nanoprobe. Results indicate that BPLP-MNPs and BPLP-Gd NPs had a size of 195 +-43 nm and 161+-55 nm, respectively and showed good stability in DI water and 10% serum for 5 days. BPLP-Gd NPs showed similar fluorescence as the original BPLP materials under UV light, whereas BPLP-MNPs showed comparatively less fluorescence. VSM and MRI confirmed that the NPs retained their magnetic properties following encapsulation within BPLP. Further, in vitro studies using HPV-7 immortalized prostate epithelial cells and human dermal fibroblasts (HDFs) showed > 70% cell viability up to 100 µg/ml NP concentration. Dose-dependent uptake of both types of NPs by PC3 and LNCaP prostate cancer cells was also observed. Thus, our results indicate that BPLP-Gd NPs would be more appropriate for use as a dual-imaging probe as the contrast agent does not mask the fluorescence of the polymer. Future studies would involve in vivo imaging following administration of BPLP-Gd NPs for biomedical applications including cancer detection.
Key Words
    nanoparticles; fluorescent; MRI; contrast agent; dual imaging
Jyothi U. Menon, Parth Jadeja, Pranjali Tambe, Dheeraj Thakore, Kytai T. Nguyen:
Bioengineering Department, The University of Texas at Arlington, Arlington, TX, USA 76019
Graduate Biomedical Engineering Program, UT Southwestern Medical Center, Dallas, TX, USA 75390
Shanrong Zhang, Masaya Takahashi: Advanced Imaging Research Center, UT Southwestern Medical Center, Dallas, TX, USA 75390
Zhiwei Xie, Jian Yang:4Department of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802

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