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Biomaterials and Biomedical Engineering Volume 3, Number 1, March 2016 , pages 59-69 DOI: https://doi.org/10.12989/bme.2016.3.1.059 |
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Conjugation of mono-sulfobetaine to alkyne-PPX films via click reaction to reduce cell adhesion |
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Hsiu-Wen Chien, Ming-Chun Keng, Hsien-Yeh Chen, Sheng-Tung Huang
and Wei-Bor Tsai
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| Abstract | ||
| A surface resisting protein adsorption and cell adhesion is highly desirable for many biomedical applications such as diagnostic devices, biosensors and blood-contacting devices. In this study, a surface conjugated with sulfobetaine molecules was fabricated via the click reaction for the anti-fouling purpose. An alkyne-containing substrate (Alkyne-PPX) was generated by chemical vapor deposition of 4-ethynyl- [2,2]paracyclophane. Azide-ended mono-sulfobetaine molecules were synthesized and then conjugated on Alkyne-PPX via the click reaction. The protein adsorption from 10% serum was reduced by 57%, while the attachment of L929 cells was reduced by 83% onto the sulfobetaine-PPX surface compared to the protein adsorption and cell adhesion on Alkyne-PPX. In conclusion, we demonstrate that conjugation of monosulfobetaine molecules via the click chemistry is an effective way for reduction of non-specific protein adsorption and cell attachment. | ||
| Key Words | ||
| anti-fouling; sulfobetaine; cell adhesion; protein adsorption; click chemistry | ||
| Address | ||
| Hsiu-Wen Chien, Ming-Chun Keng, Hsien-Yeh Chen, Wei-Bor Tsai: Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan Sheng-Tung Huang: Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Science and Technology, No. 1, Sec. 3, Chung-Hsiao E. Rd., Taipei, 106, Taiwan | ||
