Techno Press
You logged in as Techno Press

Biomaterials and Biomedical Engineering
  Volume 2, Number 1, March 2015, pages 33-45
DOI: https://doi.org/10.12989/bme.2015.2.1.033
 
open access

Fabrication of multi-well platform with electrical stimulation for efficient myogenic commitment of C2C12 cells
Joohyun Song, Eunjee A. Lee, Seungwoo Cha, Insun Kim, Yonghoon Choi and Nathaniel S. Hwang

 
Abstract
    To engineer tissue-like structures, cells are required to organize themselves into threedimensional networks that mimic the native tissue micro-architecture. Here, we present agarose-based multiwell platform incorporated with electrical stimulation to build skeletal muscle-like tissues in a facile and highly reproducible fashion. Electrical stimulation of C2C12 cells encapsulated in collagen/matrigel hydrogels facilitated the formation 3D muscle tissues. Consequently, we confirmed the transcriptional upregulations of myogenic related genes in the electrical stimulation group compared to non-stimulated control group in our multi-well 3D culture platform. Given the robust fabrication, engineered muscle tissues in multi-well platform may find their use in high-throughput biological studies drug screenings.
 
Key Words
    C2C12; electrical stimulation; agarose-based multi-well platform; collagen; matrigel; encapsulation
 
Address
Joohyun Song, Eunjee A. Lee, Seungwoo Cha, Insun Kim, Yonghoon Choi and Nathaniel S. Hwang: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea

Nathaniel S. Hwang: Institute of Chemical Process, Seoul National University, Seoul 151-742, Republic of Korea
 

Techno-Press: Publishers of international journals and conference proceedings.       Copyright © 2021 Techno Press
P.O. Box 33, Yuseong, Daejeon 305-600 Korea, Tel: +82-42-828-7996, Fax : +82-42-828-7997, Email: info@techno-press.com