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Advances in Nano Research Volume 4, Number 4, December 2016 , pages 295-307 DOI: https://doi.org/10.12989/anr.2016.4.4.295 |
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Low temperature wet-chemical synthesis of spherical hydroxyapatite nanoparticles and their in situ cytotoxicity study |
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Sudip Mondal, Apurba Dey and Umapada Pal
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| Abstract | ||
| The present research work reports a low temperature (40oC) chemical precipitation technique for synthesizing hydroxyapatite (HAp) nanoparticles of spherical morphology through a simple reaction of calcium nitrate tetrahydrate and di-ammonium hydrogen phosphate at pH 11. The crystallinity of the singlephase nanoparticles could be improved by calcinating at 600oC in air. Thermogravimetric and differential thermal analysis (TG-DTA) revealed the synthesized HAp is stable up to 1200oC. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) studies confirmed the formation of spherical nanoparticles with average size of 23.15+-2.56 nm and Ca/P ratio of 1.70. Brunauer-Emmett-Teller (BET) isotherm of the nanoparticles revealed their porous structure with average pore size of about 24.47 nm and average surface area of 78.4 m2g-1. Fourier transform infrared spectroscopy (FTIR) was used to confirm the formation of P-O, OH, C-O chemical bonds. Cytotoxicity and MTT assay on MG63 osteogenic cell lines revealed nontoxic bioactive nature of the synthesized HAp nanoparticles. | ||
| Key Words | ||
| hydroxyapatite; nanomaterials; wet chemical precipitation; biomaterials | ||
| Address | ||
| Sudip Mondal and Umapada Pal: Instituto de Física, Universidad Autónoma de Puebla, Apdo. Postal J-48, Puebla, Pue. 72570, Mexico Apurba Dey: Department of Biotechnology, National Institute of Technology Durgapur, M.G. Avenue, Durgapur-713209, Burdwan, WB, India | ||