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Advances in Nano Research
  Volume 4, Number 4, December 2016, pages 295-307
DOI: http://dx.doi.org/10.12989/anr.2016.4.4.295
 

Low temperature wet-chemical synthesis of spherical hydroxyapatite nanoparticles and their in situ cytotoxicity study
Sudip Mondal, Apurba Dey and Umapada Pal

 
Abstract     [Full Text]
    The present research work reports a low temperature (40oC) chemical precipitation technique for synthesizing hydroxyapatite (HAp) nanoparticles of spherical morphology through a simple reaction of calcium nitrate tetrahydrate and di-ammonium hydrogen phosphate at pH 11. The crystallinity of the singlephase nanoparticles could be improved by calcinating at 600oC in air. Thermogravimetric and differential thermal analysis (TG-DTA) revealed the synthesized HAp is stable up to 1200oC. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) studies confirmed the formation of spherical nanoparticles with average size of 23.15+-2.56 nm and Ca/P ratio of 1.70. Brunauer-Emmett-Teller (BET) isotherm of the nanoparticles revealed their porous structure with average pore size of about 24.47 nm and average surface area of 78.4 m2g-1. Fourier transform infrared spectroscopy (FTIR) was used to confirm the formation of P-O, OH, C-O chemical bonds. Cytotoxicity and MTT assay on MG63 osteogenic cell lines revealed nontoxic bioactive nature of the synthesized HAp nanoparticles.
 
Key Words
    hydroxyapatite; nanomaterials; wet chemical precipitation; biomaterials
 
Address
Sudip Mondal and Umapada Pal: Instituto de Física, Universidad Autónoma de Puebla, Apdo. Postal J-48, Puebla, Pue. 72570, Mexico
Apurba Dey: Department of Biotechnology, National Institute of Technology Durgapur, M.G. Avenue, Durgapur-713209, Burdwan, WB, India
 

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