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Advances in Nano Research
  Volume 4, Number 2, June 2016 , pages 145-156
DOI: https://doi.org/10.12989/anr.2016.4.2.145
 


Gadobutrol-dendrimer effects on metastatic and apoptotic gene expression
Abbas Kebriaezadeh, Sepehr Ashrafi, Rahimeh Rasouli, Seyed Esmaeil Sadat Ebrahimi, Morteza Pirali Hamedani, Artin Assadi, Mostafa Saffari and Mehdi Shafiee Ardestani

 
Abstract
    Dendrimers are one of the most appropriate nanocaries for imaging moieties in imaging applications.The purpose of this study was the evalution of cytotoxicity and inducing apoptosis of dendrimers. This study was conducted in order to investigate the metastasis suppression effect of dendrimer in human breast MCF-7 cell line and finding the nanoparticle protein corona in biological enviromental. Dendrimer cytotoxicity effect was assessed by MTT assay. The mRNA experession level of KAI1 as a metastasis suppressor gene, Bax as Pro- apoptotic gene, Bcl-2 as an anti-apoptotic gene and GAPDH as a housekepping gene were determined by real-time PCR assays.concentration-dependent nanoparticle cytotoxicity effect was proofed at range of 1-2 mg/mL in 24 hours, significant upregulation of mRNA expression of Bax, was observed whereas expression of anti-apoptotic Bcl-2 was down-regulated, also expression of metastasis suppressor gene KAI1 was up-regulated. So far a few studies confirmed apoptosis enhancement effect of dendrimers in MCF-7 cell line via bax/bcl-2 pathways. dendrimer nanoparticles was able to act as metastase inhibitor via upregulation of KAI1 gene.
 
Key Words
    dendrimer nanoparticles; MCF-7; apoptosis; cancer
 
Address
Abbas Kebriaezadeh: Departments of Toxicology/ Pharmacology and Pharmacoeconomy and Pharmaceutical Management, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Sepehr Ashrafi, Rahimeh Rasouli: Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Seyed Esmaeil Sadat Ebrahimi, Morteza Pirali Hamedani: Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Artin Assadi: Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Mostafa Saffari: Islamic Azad University Pharmaceutical Sciences Branch (IAUPS), No 99, Yakhchal, Gholhak, Shariatim
Tehran, Iran
Mehdi Shafiee Ardestani: Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
 

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