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Advances in Nano Research
  Volume 10, Number 2, February 2021 , pages 139-150

Antioxidative and antiproliferative effects of propolis-reduced silver nanoparticles
Gamze Tan, Sedef llk, Fatma Z. Foto, Egemen Foto and Necdet Saglam

    In this study, phytochemicals present in Propolis Extract (PE) were employed as reducing and stabilizing reagents to synthesize silver nanoparticles. Three propolis-reduced silver nanoparticles (P-AgNPs1-3) were synthesized using increasing amounts of PE. P-AgNPs were treated with different cancer cells-lung (A549), cervix (HeLa) and colon (WiDr) − for 24, 48 and 72 h to evaluate their anti-proliferative activities. A non-cancerous cell type (L929) was also used to test whether suppressive effects of P-AgNPs on cancer cell proliferation were due to a general cytotoxic effect. The characterization results showed that the bioactive contents in propolis successfully induced particle formation. As the amount of PE increased, the particle size decreased; however, the size distribution range expanded. The antioxidant capacity of the particles increased with increased propolis amounts. P-AgNP1 exhibited almost equal inhibitory effects across all cancer cell types; however, P-AgNP2 was more effective on HeLa cells. P-AgNPs3 showed greater inhibitory effects in almost all cancer cells compared to other NPs and pure propolis. Consequently, the biological effects of P-AgNPs were highly dependent on PE amount, NP concentration, and cell type. These results suggest that AgNPs synthesized utilizing propolis phytochemicals might serve as anti-cancer agents, providing greater efficacy against cancer cells.
Key Words
    propolis; silver nanoparticles; DPPH; SRB assay; anti-cancer activity; cytotoxicity
(1) Gamze Tan:
Department of Biology, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey
(2) Sedef llk:
Department of Immunology, Faculty of Medicine, Nigde Omer Halisdemir University, 51240 Nigde, Turkey
(3) Fatma Z. Foto:
Department of Biochemistry, Faculty of Science, Selçuk University, 42075 Konya, Turkey
(4) Egemen Foto:
Department of Biotechnology, Faculty of Science, Necmettin Erbakan University, 42060, Konya, Turkey
(5) Necdet Saglam:
Department of Nanotechnology and Nanomedicine, Institute of Science and Engineering, Hacettepe University, Beytepe, 06800 Ankara, Turkey

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