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Advances in Nano Research Volume 10, Number 2, February 2021 , pages 139-150 DOI: https://doi.org/10.12989/anr.2021.10.2.139 |
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Antioxidative and antiproliferative effects of propolis-reduced silver nanoparticles |
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Gamze Tan, Sedef llk, Fatma Z. Foto, Egemen Foto and Necdet Saglam
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| Abstract | ||
| In this study, phytochemicals present in Propolis Extract (PE) were employed as reducing and stabilizing reagents to synthesize silver nanoparticles. Three propolis-reduced silver nanoparticles (P-AgNPs1-3) were synthesized using increasing amounts of PE. P-AgNPs were treated with different cancer cells-lung (A549), cervix (HeLa) and colon (WiDr) − for 24, 48 and 72 h to evaluate their anti-proliferative activities. A non-cancerous cell type (L929) was also used to test whether suppressive effects of P-AgNPs on cancer cell proliferation were due to a general cytotoxic effect. The characterization results showed that the bioactive contents in propolis successfully induced particle formation. As the amount of PE increased, the particle size decreased; however, the size distribution range expanded. The antioxidant capacity of the particles increased with increased propolis amounts. P-AgNP1 exhibited almost equal inhibitory effects across all cancer cell types; however, P-AgNP2 was more effective on HeLa cells. P-AgNPs3 showed greater inhibitory effects in almost all cancer cells compared to other NPs and pure propolis. Consequently, the biological effects of P-AgNPs were highly dependent on PE amount, NP concentration, and cell type. These results suggest that AgNPs synthesized utilizing propolis phytochemicals might serve as anti-cancer agents, providing greater efficacy against cancer cells. | ||
| Key Words | ||
| propolis; silver nanoparticles; DPPH; SRB assay; anti-cancer activity; cytotoxicity | ||
| Address | ||
| (1) Gamze Tan: Department of Biology, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey (2) Sedef llk: Department of Immunology, Faculty of Medicine, Nigde Omer Halisdemir University, 51240 Nigde, Turkey (3) Fatma Z. Foto: Department of Biochemistry, Faculty of Science, Selçuk University, 42075 Konya, Turkey (4) Egemen Foto: Department of Biotechnology, Faculty of Science, Necmettin Erbakan University, 42060, Konya, Turkey (5) Necdet Saglam: Department of Nanotechnology and Nanomedicine, Institute of Science and Engineering, Hacettepe University, Beytepe, 06800 Ankara, Turkey | ||