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Advances in Materials Research Volume 13, Number 3, June 2024 (Special Issue) pages 233-241 DOI: https://doi.org/10.12989/amr.2024.13.3.233 |
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Quantitative analysis method for zingiber officinale water extract using high-performance liquid chromatography |
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Mohd S. Md Sarip, Nik M.A. Nik Daud, Mohd A. Mohd Zainudin, Lokman H. Ibrahim1, Syahrul A. Saidi, Zuhaili Idham and Adilah Anuar
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| Abstract | ||
| Quantitative analysis of the Zingiber Officinale sample using subcritical water extraction (SWE) was developed employing High-Performance Liquid Chromatography (HPLC) to bolster the advancement of this innovative green extraction process. This research focuses on three principal ginger bioactive compounds: 6-gingerol, 6-shagoal, and 10-gingerol. Various stages were undertaken to establish the quantitative analysis method, encompassing the optimisation of HPLC operating conditions and the formulation of standard calibration curves, yielding individual compound equations. A robust correlation within the calibration curve was achieved, exhibiting an r2 value of 0.9814 and an RSD of 5.00%. A simultaneous, swift, and dependable method was established with an injection time of 20 minutes and an 8-minute delay between injections, in contrast to the previous HPLC analysis requiring a 45-minute injection time for detecting and quantifying all components. Notably, no post-treatment was applied after the SWE process. This advancement allows for potential future online measurement of Zingiber Officinale bioactive compounds extracted using subcritical water extraction through this technology. | ||
| Key Words | ||
| 10-Gingerol; 6-Gingerol; 6-Shagoal; HPLC; zingiber officinale | ||
| Address | ||
| Mohd S. Md Sarip: 1) Faculty of Chemical Engineering and Technology, Kompleks Pusat Pengajian Jejawi 3, Universiti Malaysia Perlis (UniMAP), 02600 Arau, Perlis, Malaysia, 2) Frontier Materials Research, Centre of Excellence (FrontMate), Universiti Malaysia Perlis (UniMAP), Kampus Tetap Pauh Putra, 02600 Arau, Perlis, Malaysia Nik M.A. Nik Daud, Mohd A. Mohd Zainudin, Lokman H. Ibrahim and Adilah Anuar: Faculty of Chemical Engineering and Technology, Kompleks Pusat Pengajian Jejawi 3, Universiti Malaysia Perlis (UniMAP), 02600 Arau, Perlis, Malaysia Syahrul A. Saidi: Faculty of Electrical Engineering & Technology, UniCITI Alam Campus, Universiti Malaysia Perlis (UniMAP), 02100 Sungai Chuchuh, Padang Besar, Perlis, Malaysia Zuhaili Idham: Department of Deputy Vice-Chancellor (Research and Innovation), Universiti Teknologi Malaysia, 81310 UTM, Skudai, Johor, Malaysia | ||
